Service Testing Service Phenotyping assay

   
Phenotyping Assay
  Method  
This assay follows CLSI guideline to gate lymphocyte by CD45. The cells were stained with fluorescence labeled specific antibody. Counting beads were added to each sample immediately prior to acquisition on Flow Cytometry. Then select the CD45 Bright lymphocytes for further analysis. The absolute count or percentage of each immune cells was generated with this procedure

Target
  • T cell
  • B cell
  • Th cell
  • Tc cell
  • NK cell

   
Example
Figure A- Lymphocyte was selected for further analysis.  
Figure B- The CD3+CD4+ double positive population was helper T cells (Th cells). Th cells represented 47.46% of the lymphocyte, and counted of 683 cells /μl.  

   
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