Service Testing Service Cell Proliferation / Cytotoxicity Assay

   
Cell Proliferation / Cytotoxicity Assay
  Method  
  The effect of test article on cell proliferation or cytotoxicity is determined by treating cells with test articles in vitro.  
 
  • Detection reagents:
    • alamarBlueTM
    • WST-1
  • Peripheral Blood Mononuclear Cell (PBMC)
  • Normal cell line: fibroblast(IMR-90)
  • Cancer cell lines
    • Human hepatocellular carcinoma:Hep 3B and Hep G2
    • Human breast adenocarcinoma:MCF7
    • Human colorectal adenocarcinoma:COLO 205
    • Human lung large cell carcinoma:NCI-H460
    • Human ovary adenocarcinoma:NIH: OVCAR-3
    • Human kidney carcinoma:A-498
    • Human immune cell line: Jurkat, U-937, NK-92, K-562, CA46 and SOP-T1
 

   
Example
     
 
 
Figure A. PBMC was treated with different conc. of test article. The proliferation of PBMC was enhanced and the effect was dose-dependent.

Figure B. Jurkat cell line was treated with different conc. of test article. The proliferation of Jurkat was inhibited and the cytotoxic effect was dose-dependent.

   

   
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