The activity of phagocytosis is measured by Flow cytometry with FITC labeled E. coli.
Figure A. Distribution of cell population, gating granulocyte to analyze further.
Figure B. Phagocytosis at inactive status (4°C) as blank value
Figure C. Phagocytosis pattern under activation status (37°C)
Phagocytosis ability = percentage of 37°C - 4°